Kristen Wiig

Roboto

Brenda, Brenda Bunson

Lucy Gru / Blanche (voice), Lucy (voice), Lucy Wilde (voice), Lucy Wilde / Blanche (voice)

Maxine Simmons

Vicki St. Elmo

Aunt Carlotta

Star / Sharon Gordon Fisherman

Barbara Minerva

Jenny Hart, Denise, MayKay Bueleer

Audrey

Ruffnut (voice)

Audrey Safranek

Herald

Kelly Campbell

Erin Gilbert

Annie Montrose

Self

Narrator (voice)

Charlotte Worthington

Courtney

Funky Venice Friendette

Ruffnut (voice)

Self

Self

Patty Hearst

Agent Lucy Wilde (voice)

Ruffnut (voice)

Self

Missy

Self

Ruth Buggs

Miss Hattie (voice)

Vicki St. Elmo

Haley Sullivan

Ruffnut Thorston (voice), Ruffnut (voice)

Self

Pudgy Beaver Mom (voice)

Paulette

Surgeon

Bear Handler

Edith

Janine

Jane Whittman

Jill

Candace Van Der Shark

Young Lucille Bluth

Self - Guest

Brahbrah

Annie Crawford (voice)

Sidekick, Backup Singer (Visualized), Marion Ravenwood, Self, The Riddler, Vicky, Debbie, Pam, The Sister, Zoo Worker, Liz, Self - Host, Self - Cameo (uncredited), Kristen Wiig, Self - Various Characters, Virgania Horsen